CRISPR technology has rapidly advanced in recent years, with new tools and clinical breakthroughs. Highlight the recent developments in CRISPR-based gene editing technology applications. Discuss the opportunities and ethical challenges these advancements present for healthcare and society.

Recent development of CRISPR base gene editing technology applications 1) Vaccine development → CRISPR gene editing used for vaccine such as Malaria by ICMR. 2) Crop development - CRISPR used for development GM crops such as Kamala variety. 3) Breeder - CRISPR can be used to treat genetic diseases for neglected lowborn diseases such as Hemophilia etc. 4) Designer production of new pathogens - For production of useful items such as Insulin etc. 5) New organs - CRISPR can be used for gene silencing for new organ transplant - organ of pig in human in USA Importance 1) Easy method of gene alteration 2) Low cost of gene editing 3) Lower dark of tumor

Challenges in this technology 1) Lack of regulation - Proper legal mechanism is lacking 2) Designer babies - CRISPR can be used for creating designer bodies - case in china. 3) Germline alteration - CRISPR can be used for alteration in germline genes which can be inherited by future generation 4) Unintended impact - Creation of super weed in agriculture sector. 5) Illegal use for organ harvesting 6) Expensive - limited reach

Designer babies - CRISPR can be used for creating designer bodies - case in china. Germline alteration - CRISPR can be used for alteration in germline genes which can be inherited by future generation Unintended impact - Creation of super weed in agriculture sector. Illegal use for organ harvesting Expensive - limited reach Measures Regulation, More research to reduce cost, Better oversight and ethical use laws CRISPR holds promise to revolutionize agriculture and bio-technology. However more regulations and oversight for ethical use issued on how

— Jayant Garg · AIR 64